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Abstract Four field experiments were carried out at the Agricultural Research and Experimental Station, Faculty of Agriculture at Moshtohor, Zagazig Univ., during the two successive seasons of 1982/83 and 1983/84. -rv germination experiments were conducted in a pot trial in the same seasons. The aim of this study was to investigate the effect of gamma irradiation and some growth regulators on growth, yield and chemical content of fenugreek (Trigonella foenum gracum L.) var. Giza 30. The soil of the experiments was loam clay in texture having a pH value 7.85 and organic matter content of 2.5%. A. The first study :Two germination experiment were conducted in a pot trail during the two growing seasons. Each experiment included five seed treatments of gamma irradiation doses, i.e. 0, 1, 2, 4 and 8 K. rad. The design of the experiment was randomize complete block with four replications. Results could be summarized as follows 1. In 1982/83 season, irradiation of seeds with gamma rays had no significant effect on germination percentage. On the other hand, irradiation of seeds with 1, 4 and 8 K. rad trended to greater increase in this character in the second season. 2. Irradiation had no significant effect of germination rate index in the two successive seasons3. The irradiation of seeds with gamma rays doses of 1, 2, 4 and 8 K. rad induced significantly reduction in root length of seedling less than the unirradiated seeds in the first season only. 4. Seedling height increased significantly as the gamma doses increased up to 8 K. rad. On the other hand, number of leaves/seedling significantly decreased by increasing gamma irradiation doses in the first season only. 5. Dry weight/seedling increased as the gamma doses increased up to 1 K. rad in the two successive seasons. D The second study :Two field experiments were conducted, 9 each included 15 treatments which were the combinations of five gamma ray doses 0, 1, 2, 4 and 8 K. rad) and three IAA treatments (0, 20 and 40 ppm) or three GA3 treatments (0, 100 and 200 ppm). The treatments were arranged in a randomized complete block design. Results could be summarized as follows 1. Effect of gamma irradiation and IAA : 1. Lower radiation doses (lor 2 K. rad) have significant stimulating effect on plant height as well as dry weight per plant, whereas higher doses (4 and 8 K.rad) inhibited fengreek growth characters. At harvesting stage (160 days from sowing), number of branches/plant, number of pods/plant and seed yield/fad. significantly decreased as the gamma rays doses increased up to 8 K. rad. - 123 - Lower doses of gamma 1 or 2 K. rad increased seed yield per plant and higher doses decreased weight of seed/plant in the two seasons. o Gamma rays had no significant effect on/biolgoical yield/ fad. and chemical content of fenugreek seeds in the both seasons. 2. Effect of IAA on growth characters of fenugreek showed seasonal variation. Application of 20 or 40 ppm was more effective on plant height and dry weight/plant, respectively in the first season only. Number of pods/plant and weight of seeds/plant decreased as the IAA increased up to 40 ppm in one season, only. On the other hand, application of 40 ppm significantly increased seed yield/fad., N-content as well as Na-content in seeds over the untreated plants. IAA had no significant effect on the number of pods per plant, 100-seed weight, biological yield/fad., oil (%) , K-content and P-content in seeds. 3. Effect of the interaction between gamma rays and IAA showed significant effect on dry weight/plant, 100- seed weight, seed yield/fad., biological yield/fad., protein (%), oil (%), Na-content, K-content and Pcontent. II. Effect of gamma irradiation and GA3; 1. Plant height, dry weight/plant, number of branches per plant, number of pods/plant, lOa-seed weight, yield of seeds/plant tended to be decreased by exposing seeds to irradiation up to 4 and 8 K. rad in the both seasons. The highest seed yield was obtained at 2 K. rad dose whereas, higher doses, i.e. 4 and 8 K. rad significantly reduced the seed yield/fad. in the second season only. On the other hand, gamma ray doses had no significant effect biological yield/fad. and chemical analysis for seeds in the both seasons. 2. Number of branches/plant and number of pods/plant significantly increased by foliar application of GA 3 ppm). On the other hand, the number of branches/plant, weight of lOa-seed and oil (2) decreased by increasing GA up to 200 ppm. 3 Increasing GA3 up to 100 or 200 ppm significantly increased weight of seeds/plant than the untreated plants in one season only. The effect of GA 3 on seed yield/fad. showed no part icular trend could be detected in the two seasons. The highest yields of seed as well as biological was obtained from untreated plants with GA3 and from 200 ppm GA3 in the first and second seasons, respectively. On the other hand, GA had no significant effect on 3 plant height, dry weight/plant as well as chemical analysis for fenugreek seeds. 3. Gamma ray doses x GA had significant effect on dry 3 weight/plant, weight of 100-seed, weight of seeds/plant seed yield/fad, biological yield/fad., Na-content, Pcontent and K-content of seeds. c The third study : Two experiments were conducted, each included 12 treatments which were the combinations of three concentrations of IAA or GA and four repetations of spray- 3 ing (1, 2, 3 and 4 times). The design of the experiments was radomize complete block. Results could be summarized as follows 1. Increasing number of spraying decreased the dry weight/ plant and weight of 100-seed. Spraying plants once time by IAA or GA3 produced higher dry weight in the first season. On the other hand, increasing spraying number to 3 times with IAA and 4 times with GA significantly 3 increased the number of branches, number of pods/plant, weight of seed/plant and seed yield/fad. in one season only. whereas, spraying number showed no significant effect on plant height and biological yield/fad. 2. Dry weight/plant, number of branches/plant, N-content, A~ ”land K-content significantly increased with increasing concentrations of IAA up to 40 ppm. On the contrary, increasing concentration of IAA significantly decreased the number of pods/plant. While, plant height, weight of 100-seed, weight of seed/plant, seed yield/fad. and biological yield/fad. was not significantly affected with IAA. Plant height, dry weight, number of branches/plant, number of pods/plant, weight of 100-seed and K-content significantly incluased with increasing the GA 3 at higher concentrations. whereas, application of GA3 had no significant effect on weight of seeds/plant, and seed yield/ faddan. 3. Effect of the interaction was significant on dry weight, plant, number of branches/plant, weight of 100-seed and weight of seed/plant. |