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Abstract
The increasing world population would lead to more demands for meat and milk production therefore further increase in the number of livestock of cattle is needed since cattle is the most important agricultural animal in Egypt. So that it is very important to study the infectious agents that affect the health and productivity of cattle causing severe economic loses.
Mycoplasma is one of the causative agents of diseases to cattle which alone or together with viruses or bacteria cause important specific disorders such as pneumonia, rhinitis, infertility problems, mastitis and conjunctivitis. Respiratory diseases are the most frequently recorded causes of morbidity and mortality in cattle, therefore it is essential to identify the microorganisms responsible for respiratory diseases and to devise strategies to minimize their economic losses.
The aim of the present work was the isolation and identification of Mycoplasma from cattle suffering from respiratory diseases by conventional methods and applying recent techniques as PCR, RAPD and IHC on the obtained isolates and interpretation of the advantages and disadvantages of each diagnostic method.
In this study, a total of 520 samples from apparently healthy and diseased cattle were .