الفهرس 
Material and methodsOnly 14 pages are availabe for public view
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Abstract
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This study was designed to determine the T-helper cell subsets in
severe asthmatic children and ’to evaluate the effect of treatment with
prednisolon for one week on it and on calculated Th1ITh2 ratio
The study included 20 patients with severe asthma considered as
study group and 10 healthy children of cross-matched age and sex and
gave blood sample considered as control samples. After through clinical
examination, chest X-ray examination, routine laboratory investigation
included complete blood picture and erythrocyte sedimentation rate.:
All the study participants g. ave two. samples:.pre-treatment and on
the -,.t\’” day after treatment (Post-treatment) for flow cytometric
determination of T-helper cell subsets; namely: Pre-T-helper cell (Tho),
T-helper cell type 1 (Th1), T-helper cell type 2. (Th2) and the ratio of
Th11Th2•
Eight patients were symptomatizing since birth, 4 patients at age
younger than one year, in other 4 patients at age of one year and in 2
patients at age of 2 years; while in the other 2 patients disease ’was
presented at age of 4 years.
Only one patient (5%) had disease duration of 3 years; while 12
patients (60%) had mean disease duration of 7.7±0.8; range: 6.3-9
years and the other 7 patients (35%) had diseased since a mean
duration of 12.3±1.5; range: 10-14 years.
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In control group; no APC could be detected in 4 controls (40%)
and other controls had <100 cell/cc, while 15 patients had APC >100-
500 cells/cc; while another 5 patients had >500 APC cellsicc with a
significant increase of frequency of detection of APC in patients
compared to controls’, (X2=9.15, P<0.001).
The percentage of CD4 in pre-treatment samples showed a nonsignificant
(P>0.05) decrease compared to control levels. Furthermore,
post-treatment samples showed a non-significant (P<0.05) decrease of
percentage of CD4 compared both to control and pre-treatment levels.
There was a significant (P<0.05) decrease of percentage of Tho in
patients both pre-treatment and post-treatment samples compared to
control levels with a significant (P<O.05) decrease of the percentage of
Tho in pre-treatment compared to post-treatment samples.
There was a significant (P<O.05) decrease of percentage of Th1 in
patients both pre-treatment and post-treatment’ samples compared to
control levels with a non-significant (P>0.05) difference between
percentage of Th1 between pre-treatment and post-treatment samples.
There was a significant (P<O.05) increase of percentage of Th2 in
pre-treatment sample compared to both control and post-treatment
levels with a non-sig!”ificant (P>O.05) difference between percentage of
Th2 cell in post-treatment samples compared to control samples.
There was a significant (P<O.05) decrease of Th1/Th2 in patients;
pre-treatment and post-treatment samples, Compared to control levels.
Moreover, there was a significant (P<O.05) increase of the Th1/Th2 ratio
in post-treatment samples compared to pre-treatment samples.