الفهرس | يوجد فقط 14 صفحة متاحة للعرض العام |
المستخلص This study was carried out in an attempt to throw more light on the effect of cadmium administration on the pancreas and parotid gland of albino rat. Also, to study the possible protective role of vitamin C (ascorbic acid). Forty young adult albino rats weighing from 90 -110 gms in weight were used in the present study. They were weighed and the blood sugar was measured weekly. The animals were randomly assigned to four groups each of 10 rats as follows: Group I (Normal control group): It is composed of 10 rats. Each one was administered 0.5ml physiological saline solution daily orally by a modified plastic syringe for one month. Group II (Ascorbic acid treated group): It is composed of 10 rats .They received a daily dose of vitamin C (20 mg /kg) dissolved in 0.5ml physiological saline solution daily orally for a period of one month. Group III (Cadmium chloride treated group): It is composed of 10 rats .They received a daily dose of cadmium chloride (2 mg /kg) dissolved in 0.5ml physiological saline solution daily orally for a period of one month. Group IV (Protected group): It is composed of 10 rats .They received a daily dose of cadmium chloride (2 mg /kg) dissolved in 0.5ml physiological saline solution daily orally simultaneously with vitamin C (20 mg /kg) for a period of one month. Rats were checked for base line blood glucose level on the day before the start of the experiment [day 0]. In all groups, blood glucose was measured on days 0, 7, 14, 21 and 30(day of scarification). Blood samples were obtained at 10 a.m. from the rat dorsal vein by tail snipping of non-fasting animals to determine blood glucose using a glucometer and strips. The average weight of the animals was recorded on the same days. At the time of scarification, animals were killed by decapitation. The whole pancreas and the two parotid glands were quickly dissected, excised then blotted. The tissue was subdivided into two parts (light and electron microscopic examination).For light microscopy, immediate fixation of the pancreas and parotid glands in either 10% formalin or 10% formol saline was done for 5-7 days. Fixation, dehydration, clearing and embedding in paraffin blocks were done. Paraffin blocks prepared from specimens originally fixed in formalin were cut into 4-6 um thick sections and stained with the following stains : 1- Haematoxylin and Eosin stain H. &E. 2- Masson’s trichrome stain MT 3- Periodic acid-Schiff’s reaction PAS |