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Abstract
This thesis is concerned with the spectrophotometric and spectroftuorimetric analysis of some psychoactive drugs namely; chlorpromazine HCI, thioridazine HC1, haloperidol, DROPeridol, imipramine HCI, desipramine HCI and clomipramine HCI. The thesis comprises three parts in addition to general introduction about antipsychotic drugs and different reported methods described for assay of these drugs. Parti
A simple, rapid, accurate and sensitive extractive spectrophoto¬metric method was developed for determination of the studied psychoactive drugs. The method is based on the reaction of each drug with oxidized quercetin at pH range from 4.5 to 5.0 to form ion associate complex. The colored complex was quantitatively extracted into dichloroethane and measured in the range of 528-535 nm. A study for all the reaction variables has been conducted to optimize the reaction conditions. Also a validation study for the proposed procedure according to USP 2002 was performed. Beer’s law was obeyed for all the studied drugs in the concentration range of 2.0 - 50.0 jig/ml. The detection limit range was 0.80 -1.14 (ig/ml while the quantitation limit range was 2.68 -3.79 ug/ml.
The method was successfully applied to the analysis of studied drugs in pure form and in commercial Pharmaceuticals. Results were compared with those obtained from the official or reported methods. The t- and F- values were calculated and compared with the theoretical values, indicating high accuracy and good precision of the proposed procedure.
out according to USP 2002. Linearity range was 0.05 - 1.3 jig/ml. The limit of detection range was 0.03 - 0.04 u.g/ml while the limit of quantitation range was 0.12 - 0.13 jxg/ml. The method was successfully applied for assay of the studied drugs in pure form and in pharmaceutical dosage forms. Results were compared with official methods. The t- and F- values were calculated and compared with the theoretical values, which indicate high accuracy and good precision of the proposed method.
Part II
This part is concerned with the development of simple, rapid and
I accurate kinetic spectrophotometric method for determination of
Ichlorpromazine HC1, thioridazine HC1, clomipramine HCI, imipramine
I HCI and desipramine HCI. The method is based on in-situ oxidation of
I quercetin into a red colored product measured at 515 nm. The reaction
I rate was followed by measuring the decrease in absorption intensity
caused by the investigated drugs. Investigations were carried out to study
I the reaction variables, effect of commonly encountered excipients.
Validation of the proposed procedure according to USP 2002 was also
carried out. Beer’s law was obeyed for all the studied drugs in the
concentration range of 5.0 - 50.0 fig/ml using slope, fixed time and
variable time methods. The detection limit range was 1.33 -2.14 |ig/ml
while the quantitation limit range was 4.444 -7.15 jag/ml. Fixed time
method was successfully applied to the determination of studied drugs
either in pure form or in their pharmaceutical preparations.
Part ITT
In this part, five psychoactive drugs namely, chlorpromazine HCI,
thioridazine HCI, clomipramine HCI, imipramine HCI and desipramine HCI were analyzed by a simple spectrofluorimetric method. The method is based on oxidation of the studied drugs using cerium (IV) in presence of sulphuric acid and monitoring the fluorescence of cerium (III) formed at ^x- 254 nm. and Xcm.= 355 nm. All variables affecting the reaction conditions such as; cerium (IV) concentration, sulphuric acid concentration, heating time, temperature and dilution solvents were carefully studied. The effect of potential interference due to common ingredients as glucose, sucrose, lactose, citric acid and propylene glycol were investigated. A validation study of the proposed method was carried