الفهرس 
material and methodsOnly 14 pages are availabe for public view
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Abstract
The present study began with 80 adult albino rats of both sexes (60 females and 20 males). The animals were divided into 4 equal
groups each comprising 15 females and 5 males. The first group served
as controls and its animals were injected with distelled water, the
remaining groups served as experimentals as follows:-
- Females and males of the first experimental group were injected
with a freshly prepared aqueous solution of the diabetogenic agent
Streptozotocin in a single dose of 40mglkg body weight one week
before mating.
- Only females of the second experimental group were injected
with the diabetogenic agent.
- Only males of the third experimental group were injected with
Streptozotocin.
The offspring of each group were further subdivided into 3
subgroups (A, 8 and C) according to their ages (2, 4 and 12 weeks
respectively). As well, and according to the random blood glucose level,
the offspring were divided into diabetic (200 mg/dL or more), nondiabetic
hyperglycemic (> 120 <200 rng/dl.) and non-diabetic normal «
120 mg/dL).
For cach animal, biochcmical investigation (blood glucose
conccntration measurement), chromosomal study (direct metaphase
smears from bone marrow cells), mitotic index evaluation, histological
study (morphological and morphomctric) and quantitative study
(statistical analysis of the volumetric data using the t distribution test
and the Z test) were done.
The results showcd the following:
- SZ was able to induce significant and irreversible hyperglycemia in all
injected adult animals.
- The offspring of diabetic mothers (expcrimental groups I and II)
exhibited hypoglycemia during the early weeks of postnatal life (age of
2 weeks) and then hypcrglycemia from the age of 4 weeks and
thenccaOcr.
- The offspring of experimental group III were analogous to their age
corresponding controls.
- The chromosomal anomalies were virtually higher in diabetic
subgroups but this increase in the rate of abnormalities was statistically
non-signi ficant.
- The diabetic offsprings (blood glucose > 200 mg/dl.) exhibited
signiflcantly lower proliferation indices. However, non-diabetic
hyperglycemic offsprings showed non-significant differences from their
age corresponding controls.
- Histological changes concerning fl and a-cells were directly
proportional with the level of blood glucose. Beta cells showed
pyknotic and karyorrhexic nuclei, hyDROPic degenerative changes in
their cytoplasm, and lastly ballooning and complete loss of the cell.
Alpha cells, on the other hand, showed extensive eosinophilia with
diabetic animals; indicating accumulation of secretion in their
cytoplasm.
- Morphometric study of J3-cells showed that their nuclei were enlarged
significantly in a trial cope with the state of hyperglycemia. Finally, the
cells were exhausted and their nuclei showed the different stages of
degeneration.
III COilC/usioll, the present investigation provided an evidence
that hyperglycemic milieu found surrounding the foetuses during their
intrauterine life might affect their J3-cells colonies and result in the
production of endocrine pancreatic mass which is unelTicient in
producing the required amounts of insulin. However, paternal diabetes
was found to be of negligible effect. Accordingly maternaillealth and
care of prediabeties and diabeties must take a prime importance in its
regulation and control during the earliest weeks of pregnancy or
preceding pregnancy if possible.