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Abstract
100 random samples of roast beef were collected from food services establishments under examination in Kaft Elsheikh governorate and subjected to the enumeration of total mesophylic bacterial count and Staphylococcal count, as well as isolation and identification of isolated was carried out. The mean value of total mesophilic bacterial count of examined roast beef samples was I .87x102±O. I 78x1 02 cfli/g, regarding Enterobacteriaceae the mean value was 1 .97x I 0±0.4 1 2x 1 o cfulg and the mean value of total Staphylococcal count was 4.03x104±0.37x104 cfulg.
The identified enteric bacteria isolated from examined roast beef samples were:
E.coli 19 (19%), Citdiversus 2(2%), Enterobacter agglomerans 13 (13%), Ent.cloacal 2 (2%), Ent.gerogvial 2(2%), Klb.oxytoca 2(2%) and Klb.pneumoniae 1(1%).
Serotyping of E.coli isolated from the examined samples of roast beef was declared in Table (10). Accurately, out of ig strains of E.coli were serologically identified as 026:Koc (B6), 086:1(61 (B17), Oi :K58 (Bg), 0124:K72 (B17), 0128:K67 (B12) and untypable at an incidence of 2(2%), 3(3%), 2(2%), 5(5%), 3(3%) and 4(4%), respectively.
The isolated Staph aureus from samples was 42 (85.7%) coagulase positive and 7 (13.7%) coagulase negative.
Salmonella could not be detected in all roast beef samples under this study. Yersinia spp. were isolated with incidence of Y.enterocolitica 10 (10%), Y.pestis 20 (20%) and Y.enteromedia 8 (8%).
The identified isolated Y.enterocolitica biotypes isolated from examined roast beef were: IA, IB, 2, 3, 4 and 5 with incidence of 2 (20%), 2 (20%), 1 (10%), 2 (20%), 1 (10%) and 2 (20%) respectively.