الفهرس 
Chapter I : IntroductionOnly 14 pages are availabe for public view
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Abstract
STUDIES ON SHEEPPOX VIRUS
This research aimed to isolate and recognize SPV via sheep herds and the research investigate the following:
1- The affected sheep show clinical signs like nodules, scars and crusts which are obviously appeared.
2- Samples from these affected animals were collected in order to isolate and recognize the SPV with normal serological and non serological means.
3- In order to make preliminary identification of the SPV antigen using AGPT on skin samples.
4- In order to isolateSPV:
The prepared samples from skin lesions were inoculated in embryonated chicken eggs by the CAM route which shown reddness and oedema of the inoculated CAM without death of theembryo.
5- The inoculated CAM were harvested and AGPT and IFAT tests were done which gave positive results for viral antigen and IFAT revealed that the virus present in the cytoplasm of CAM cells.
6- PCR test was done using known primer on harvested samples from CAM.Amplification and running of characteristic fragments of SPV DNA on Ethidium Bromide Stained Agarose Gel, And the DNA of referance Kenyan SPV strain.then staining of both DNA gave typicalresults in many segments on DNA fragment. Which indicate that the given sample contain SPV antigen.
7-Trial for identification of the biological properties of the virus revealed that:
- The isolated virus had haemagglutinating property to many RBCs either avian or mammalian RBCs.
- The results revealed that the virus is non eluting virus.
- The results showed that the virus is cell associated virus.where as HA did not occur except with direct prepared CAM and not occurred after centrifugation to CAM which ensure absence of the virus.
- The resuls showed that HA test best temperature at 4 C for all used RBCs.
- For more studying of physical properties of the virus. The virus was treated with ultra- violet radiation for 20minutes.HA tests revealed that the virus severly affected when exposed to these radiations.
** from the previous we can say that the isolated virus from affected animals (sheep) assumed to be SPV. Is SPV and AGPT is very sensitive as IFAT do and as PCR had done.
• The SPV cause haemagglutination to RBCs and not eluting virus.
• The isolated virus is cell associated virus and easily affected by U/V radiation. Which must be put inyo consideration when dealing with the virus.
• The isolated virus is typically as Kenyan strain which used as marker for identification of SPV in Egypt. Which used in many researches but the physical and biological similarity not sudied yet which must be taken into consideration in the future.
STUDIES ON SHEEPPOX VIRUS
This research aimed to isolate and recognize SPV via sheep herds and the research investigate the following:
1- The affected sheep show clinical signs like nodules, scars and crusts which are obviously appeared.
2- Samples from these affected animals were collected in order to isolate and recognize the SPV with normal serological and non serological means.
3- In order to make preliminary identification of the SPV antigen using AGPT on skin samples.
4- In order to isolateSPV:
The prepared samples from skin lesions were inoculated in embryonated chicken eggs by the CAM route which shown reddness and oedema of the inoculated CAM without death of theembryo.
5- The inoculated CAM were harvested and AGPT and IFAT tests were done which gave positive results for viral antigen and IFAT revealed that the virus present in the cytoplasm of CAM cells.
6- PCR test was done using known primer on harvested samples from CAM.Amplification and running of characteristic fragments of SPV DNA on Ethidium Bromide Stained Agarose Gel, And the DNA of referance Kenyan SPV strain.then staining of both DNA gave typicalresults in many segments on DNA fragment. Which indicate that the given sample contain SPV antigen.
7-Trial for identification of the biological properties of the virus revealed that:
- The isolated virus had haemagglutinating property to many RBCs either avian or mammalian RBCs.
- The results revealed that the virus is non eluting virus.
- The results showed that the virus is cell associated virus.where as HA did not occur except with direct prepared CAM and not occurred after centrifugation to CAM which ensure absence of the virus.
- The resuls showed that HA test best temperature at 4 C for all used RBCs.
- For more studying of physical properties of the virus. The virus was treated with ultra- violet radiation for 20minutes.HA tests revealed that the virus severly affected when exposed to these radiations.
** from the previous we can say that the isolated virus from affected animals (sheep) assumed to be SPV. Is SPV and AGPT is very sensitive as IFAT do and as PCR had done.
• The SPV cause haemagglutination to RBCs and not eluting virus.
• The isolated virus is cell associated virus and easily affected by U/V radiation. Which must be put inyo consideration when dealing with the virus.
• The isolated virus is typically as Kenyan strain which used as marker for identification of SPV in Egypt. Which used in many researches but the physical and biological similarity not sudied yet which must be taken into consideration in the future.