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Abstract Abstract A total number of 108 raw milk samples were collected from different localities in Ismailia City, 72 samples were collected from markets (36 samples from dairy shops and 36 samples from street vendors), in addition to 36 samples collected from individual dairy buffaloes in a governmental farm. The collected samples were subjected to the bacteriological examination, which revealed the following results : Enterobacteriaceae organisms, represented by Escherichia coli, Citrobacter species, Klebsiella species, Enterobacter species, Salmonella species, Proteus species and Morganella morgani,as well as Pseudomonas species, Staphylococcus species, Streptococcus faecalis and Coryneforms were recovered with percentage of 8.33%, 25%, 19.44% , 30.55%,5.55%, 12.50% 2.78%,12.50%, 5.55%, 16.67% & 30.55% of the examined market raw milk samples respectively and 2.78%, 2.78% ,0%, 2.78%, 0%, 0% 0%, 13.89%, 47.22%, 2.78% & 16.67% of the examined farm milk samples respectively , with total isolation of 6.48%, 17.59%, 12.96%, 21.29% , 3.70%, 8.33% , 1.85%, 12.96%, 19.44%, 12.04% & 25.92% of the total examined raw milk samples respectively . Serotyping of the isolated Escherichia coli strains from the examined raw milk samples revealed identification of Escherichia coli O125 poly1 (one strain) , Escherichia coli O128 poly1 (one strain) & Escherichia coli O- poly 3 (one strain) , while 4 strains could not be serotyped . Serotyping of the isolated Salmonella strains from the examined market raw milk samples showed identification of Salmonella paratyphi ”C” (one strain), Salmonella typhimurium (2 strains) and Salmonella enteritidis (one strain) . Key words Raw milk – Pathogenic bacteria |