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Abstract
The present study was designed to test the efficiency of colchicine in inducing polyploidy in two floricultural plants using different methods of application, concentrations and time of application, The two tested plants were Antirrhinum majus cv. Rose Bicolor and Delphinium ajacis cv. Cliveden Beauty. The investigated parameters were emergence rate, stem length, stem thickness, leaf characteristics, stomata characteristics, flower characteristics, pollen grain characteristics and fertility, chlorophyll a, chlorophyll b, chromosome number, and DNA content. Results revealed that soaking seeds in 1% colchicine and 24 hours operating time induced the highest number of tetraploid plants in Antirrhinum majus, while 0.5 % for 48 hours gave the same result in Delphinium ajacis. Expression of gigantism in morphological characters such as plant height, size of leaves, flowers, seeds and pollen grain diameter were observed in tetraploid plants, with significant decrease in stomatal frequency and pollen fertility.