الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
 |  | from | 128 |  |  |
| | | from | 128 | | |
Abstract
. I- In vitro Several methods were used for the determination of quinoline group of antibiotics. These methods were agar diffusion test,
high voltage electrophoresis and high performance liquid chromatography. For the agar diffusion test, E.coli strain 14 Bayer and standard II agar were used. The detection limits of the reference substance solutions were 0.05, 0.035, 0.1, and 1 0 g/ml for flumequine, baytrilR, oxolinic acid and nalidixic acid respectively. It was found that, these detection limits were lower than the detection limits of the same reference substance solutions by using the German standard inhibition
test. By using high voltage electrophoresis, it was found that, 1.:. both flumequine and baytrilR were migrated toward the cathode
side with a detection limits of 0.2 and 0.1 g/ml respectively. ;. he detection limit of flumequine reference substance solution
y using HPLC combined with fluorescence detector was 0.035 g/ml and in serum was 0.1 g/ml serum. The extraction of
amples was performed with acidified chloroform. The recovery * ate of flumequine from serum in vitro was 56.3% and from uscles and organs ranged from 49.32 to 53.7%.
I- Experimental
164 healthy laying hens were used in this study and classified as follows.
Single dose study. A total of 82 laying hens was classified
into 5 groups. The 1st and 2nd groups (26 each) were applied a
single i.m. and oral dose of flumequine at 12 mg/kg respectively for determination of flumequine in serum, as well
as its residual content in muscles and internal organs. The 34 and 4th (10 each) groups were given a single i.m. and oral dose of flumequine at 12 mg/kg respectively for determination of the residual pattern of the drug in the eggs. The 5th group (10 hens) were used as a negative control. Flumequine could be detected in serum of laying hens until 24 and 36 hours after a single i.m. and oral application respectively. The maximal mean concentration of flumequine in serum (11 ± 0.63 and 3.4 ± 0.31) were obtained one and 3 hours after i.m. and oral application resspectively. Fluxnequine could be detected in faeces until 72
hours in case of i.m. and oral applications respectively. 4 Flumequine was detected in all muscles and organs of laying hens 3, 6, 12, 18, 24 and only in the lung and kidney until 36 hours of a single i.m. dose. While after oral application, flumequine could be detected in all muscles and organs 3, 6, 12, 18, 24 and 36 hours. The systemic bioavilability of flumequine after i.m. administration was higher than after oral administration. A lag period was found between the appearance of flumequine in egg white and egg yolk, also this lag period was found between the disappearance of flumequine from the.egg white and egg yolk. This lag period was found to be 5 days.