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Abstract
Ergoline alkaloids (chanoclavine-I, lysergic acid amide or ergometrine) were not detected by TLC, HPLC or capillary electrophoresis (CE) in Convolvulaceae (Rivea corymbosa, Ipomoea asarifolia or Ipomoea violacea) plant tissue cultures grown under different cultural conditions, while their corresponding intact plants grown under field conditions contained ergoline alkaloids. Many endophytic fungi have been isolated from R. corymbosa leaf and stem. 3 fungal isolates from I. asarifolia were resistant to the fungicide Benomyl* and Switch* (Iasarsw-01, -02 and Penicillium roquefortii). By sequence analysis of the ITS regions of Iasarsw-01 and Iasarsw-02, they were identified as Bjerkandera fumosa and Penicillium roquefortii respectively. Ergoline alkaloids were detected by using TLC and CE in the submerged cultures of some of these fungi. The results obtained from this work suggest the close association between the presence of endophytic fungi and the production of ergoline alkaloids in Convolvulaceae