الفهرس Only 14 pages are availabe for public view
 |  | from | 159 |  |  |
| | | from | 159 | | |
Abstract
Introduction: Expanding stem cell precursors using
different culture media and supporting endothelial cells has been shown in previous studies to result in grafts that are capable of hematopoiesis in myeloablated host. The use of xenogenic endothelium or cell lines is not applicable to humans without concern.
Patients and methods: We investigated the feasibility
of ex vivo expansion of cord blood stem cells by co-culturing with autologus HUVEC or placenta in the presence of 3 or 5 growth factor cytokine cocktails. We compared the fold increase in expansion to the control arm of the study; the cord stem cells in liquid cultures with added cytokine cocktail. HPC, CD34+ cells,
placenta and HUVEC were all isolated from the same umbilical cord. Results: Total cells, CD34 and CFU were significantly increased when cultured with the HUVEC or placenta compared to the liquid cultures. In the liquid cultures, five growth factor cytokine cocktails lead to an expansion that was significantly more than that observed using three cytokines only but less than the co culture arms.
Conclusion: The use of autologus derived HUVEC or placenta provides an exciting and potentially clinically applicable approach to expand the umbilical cord stem cells for multiple uses in both the pediatric and adult patient, superior to the cytokine supplemented liquid cultures.