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Abstract
Brucellosis is primarily a disease of domestic animals causing abortion so it is considered one of the most serious of 11w current public health problems. Brucellosis in Egypt is still one of the most serious problems facing animal production so this placed it on the top priorities for control programs of Brucella.
A total of 608 animal and human samples were collected from (liflercrlt localities in Behera. and Alexandria Govci’norales. ‘Serum samples were collected from cattle and sheep in the following respective numbers; cattle 114 and sheep 74 from ln(Iivi(Iually owned animals, in addition to 10 whole blood sam pies with EDTA were collected from serologically positive cattle to be tested by PCR. Moreover, 100 milk samples were collected, 50 each of cow and buffalo milk. The collected samples were examined by BAPAT and RBPT and positive samples were confirmed by Riv.T and TAT while milk samples were tested 1w
MRT.
Concerning human samples, a total of 300 serum samples were collected at the rate of 75 sample per season from fever hospitals fi’oin patients suffering from fever of unknown origin. These collected samples were examined by BAPAT and RBPT and positive samples were confirmed by Riv.T and TAT, in addition to 1 0 whole blood samples with EDTA were collected from serologically positive patients to be tested by PCR.