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Abstract
Pullorum disease (PD) and fowl typhoid are septicaemic diseases and can be transmitted through egg, also Mycoplasma gallisepticum cause lowering egg production and downgrading of carcasses. So this work study some serological tests for rapid diagnosis of previous bacterial diseases and the obtained results can be summarized as following:
Eight hundred laying hens in four different flocks of local breeds were examined by pullorum test and 120 hens were found to be pullorum test positive with percentage 1.5% of total number of examined samples. The examined 4 flocks as follows:
Four hundred layers of Matroh local breed, 53 hens were pullorum test positive with percentage 13.25%.
On the other hand, 800 examined Dokki-4 laying hens; from it 10 layers were proved to be pullorum test positive with percentage of 1.25%. Also by examined 6800 samples Fayoumy breeds in two flocks each 3400 layers, in the first flock found 31 layers were positive pullorum test with percentage (0.91%) while other flock of 26 laying hens were positive pullorum test with percentage (0.76%).
On the other hand out of 120 pullorum test positive sera, 105 (87.5%)
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serum samples were found positive by using tube agglutination test.
Out of the positive sera 39 serum samples were belonged to Matroh flock, 10 sera were belonged to Dokki-4 flock and 56 serum samples were belonged to Fayoumy flocks.
Nine serum samples showed 1/40 (8.57%), 10 showed 1/80 (9.5%), 15 of 1/160 (14.28%), 24 of 1/320 (22.85%) and 47 of 1/640 (44.76%).
More over, 9 cases revealed suspicious titer 1/20 with an incidence of 7.5%. The percentage of negative sera was 5.0% of the total number of examined samples.
Using rapid plate agglutination test and Mycoplama gallisepticum colored antigen for examined 400 serum samples collected randomly from four flocks under study the result indicated that 254 serum samples (63.5%) were found positive divided as follows Matroh flock examined 100 samples 64 were positive (64.0%) from Dokki-4 only 18 samples were positive. Mean while, 172 out of 200 sera collected from two flocks of Fayoumy breed.
Ninety-one positive sera when examined by slide agglutination test were selected and examined serologically with ELISA which divided as follows 46 (Fayoumy flocks), 28 (Matroh flock), 17 (Dokki-4 flock) from the sera proved to be positive with plate agglutination test using Mycoplasma gallinsepticum colored antigen.
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The results as follows:
Seventy-three samples were positive divided as:
Thirty-eight (84.8%) of the examined 46 sera Fayoumy flocks were 22 (78.6%) of 28 sera Matrroh flock, and 13 (76.5%) of 17 Dokki-4 flock reacted positive with ELISA coating antigen.
ELISA test for 91 samples of extracted yolks for detection of IgY antibodies from 52 sera this samples were positive divided as follows 3 Fayoumy from it 17 (51.5%) were positive, 29 Matroh flock from it 15 (51.7%) were positive and 29 Dokki-4 from it 20 (68.96%) were proved to be ELISA positive.
Studied experimental infection with a reference strain of M.gallisepticum on local and foreign breed of chickens by different routes with or without inoculation of MG oil adjuvant vaccine and IgY-positive yolks.
The experimental infection of 120 local breed chickens of 21 days old divided 6 groups each group of 20 birds:
The 1st group S/C inoculated with 0.2ml/bird of 1x10¬6 CFU/ml PPLO broth of M.gallisepticum PG31 strain recorded 10.0% mortality at days 12 and 17 post infection with 5 positive serum samples to M.gallisepticum using slide agglutination test from 18 examined sera.
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The 2nd group intratracheally instillated group recorded 15.0% mortality at 10 and 13 days post infection with 6 of 17 examined sera were positive using slide agglutinatintion test.
The 3rd injected S/C with M.gallisepticum as well as with MG oil adjuvant vaccine recorded 10% mortality at days 13 and 14 post injection with 8 of 18 examined sera were positive by slide agglutination test.
The 4th group inoculated S/C with 0.2 ml of each of M.gallisepticum and IgY positive yolk diluted recorded 5% mortality at 12 days post infection with 7 of 19 examined sera were positive by slide agglutination test.
The 5th group intratracheally inoculated with 0.2 ml of PPLO broth containing 1x106 CFU/ml of MGPG31 with S/C inoculation of 0.2ml of IgY positive yolk recorded 10% mortality at 13 days post inoculation with 7 of 18 serum samples were positive by slide agglutination test.
Control group recorded 5% mortality at 19 days of infection with 3 positive serum samples by slide agglutination test.
As regards to Cobb foreign breed 120 broilers experimentally infected at one-day old, divided to 6 groups of 20 birds as follows.
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The 1st group S/C inoculated with M.gallisepticum recorded 10% mortality at days 16 and 17 post infection with 9 of 18 examined sera were positive with slide agglutination test and only 3 serum samples recorded positive with ELISA.
The 2nd group intratracheally instillated with M.gallisepticum recorded mortality 20% at 12 days post infection with 11 of 16 examined sera were positive with slide agglutination test, and only 3 positive serum samples with ELISA.
The 3rd group injected S/c with M.gallisepticum and oil adjuvant MG. vaccine recorded 10% mortality at 21 days post injection with 12 of 18 examined sera were positive by plate agglutination test and 2 serum samples were positive with ELISA test.
The 4th group injected S/C with MG. and IgY positive yolk diluted recorded 10% mortality at 20 days post injection and 5% mortality at 21 days post injection.
The 5th group instillated intratracheally with M.gallisepticum and injected by IgY-positive yolk. Recorded mortality 15% at days 9, 11 and 19 post instillation with 12 of 17 examined sera were positive serum samples.