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Abstract
A trial was made to isolate S. aureus from uterine discharge suffering from endometeritis, metritis, toxic shock syndrome, retained placenta and pyometra.
Results showed that the occurrence of S. aureus in human samples and animal samples were 11.9 and 12.3%, respectively.
Also, the results revealed that the most incidence of S. aureus during post labour discharge was highest (53.8%) in human samples which showed toxic shock syndrome than prelab our and normal uterine discharge in a percentage of (30.7 and 15.3%, respectively). Also, the results revealed that the most incidence of S. aureus during post labour discharge was highest (41.6%) in animal samples which showed endometeritis, metritis, retained placenta and prolapse of vagina than other discharges as prelabour .rand normal uterine discharge a percentage of (33.3 and 25%, respectively).
Results of antimicrobial sensitivity test from human samples revealed that S. aureus isolates were highly sensitive to ciprofioxacin, gentamicin and doxycycline (100, 66.6 and 45%, respectively). But they were highly resistant to penicillin G, ampicillin and amoxicillin (100, 91.6 and 83.3%, respectively).
Results of antimicrobial sensitivity test from animal samples revealed that S. aureus isolate were highly sensitive to ciprofioxacin and gentamicin (88.8 and 88.8%, respectively). They were highly resistant to penicillin G, ampicillin, amoxicillin and doxycycline (100, 100, 88.8 and 77.7%, respectively).
PCR technique was used as a rapid, sensitive and specific tool to detect S. aureus. The chosen 5 samples were tested then they were tested with using multiplex PCR with the specific primers of S. aureus. Revealed that PCR detected all samples of S. aureus with positive bacteriological examination results.